Although nitrogen application could improve growth and yield of roselle, fertilizer recommendations for Niger are lacking. The objective of this study was to evaluate the effects of two treatments of nitrogen (N1=50 kg N,ha-1 and N2=100 kg N.ha-1) on yields and yield components in three ecotypes of Roselle (A3, A7 and A9). An increase in nitrogen was associated with an increase in leaf yield in every ecotype. However, there was no significant difference in yields between N1 and N2. Compared with the control plants (N0), the increase was about 181& for A3, 70& for A7 and 95& for A9 at N2. This level of nitrogen significantly decreased seed yield by 30& for A3 and 48&, respectively for A7 and A9. Nitrogen treatment had no effect on calyx yield which was approximately identical for the three ecotype (420 kg ha-1). With the exception of the number of branches/plant, yield components were not affected by nitrogen application. Therefore, N1 could be considered as the optimum fertilization for leaf yield.